Incubation:Article Title: Interleukin 33-mediated inhibition of A-type K + channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice
Article Snippet: To increase the specificity of the ST2 antibody, the ST2 antibody was preadsorbed against its blocking peptide (Sigma, Cat. No. SBP3363) prior to incubation with DRG sections, by mixing the ST2 antibody with a five-fold (by weight) of the blocking peptide. .. After washing with PBS, the sections were incubated for 1 h at room temperature with the appropriate secondary antibodies: Alexa Fluor 555 goat anti-rabbit (1:300, Cell Signaling Technology, Cat. No. #4413) and Alexa Fluor 488 goat anti-mouse (1:300, Cell Signaling Technology, Cat. No. #4408). .. All images were acquired using a Nikon 104c fluorescence microscope.
Article Title: Alcohol Consumption Accumulation of Monocyte Derived Macrophages in Female Mice Liver Is Interferon Alpha Receptor Dependent
Article Snippet: Nonspecific reactions were blocked with 5% normal goat serum (ab7481, Abcam, Cambridge, MA) and 5% TruStain fcX (BioLegend, San Diego, CA) in PBS-T for 1 h and then incubated with rabbit anti-mouse F4/80 (1:200, ab111101, Abcam) and rat anti-mouse Ly-6C (1:200, ab24973, Abcam) at 4°C overnight. .. After washing in PBS-T, the specimens were incubated with Alexa Fluor 488 goat anti-rat IgG (H+L) (1:400, #4416, Cell Signaling, Danvers, MA) and Alexa Fluor 555 goat anti-rabbit (1:400 #4413, Cell Signaling) for 1 hour at room temperature, washed again, treated with Vector TrueVIEW Autofluorescence Quenching Reagent and then counterstained with VECTASHIELD Mounting Medium with 4’,6-diamino-2-phenylindole (DAPI) (both: Vector Labs, Burlingame, CA). .. The Zeiss Axio Observer Microscope (Carl Zeiss Micro Imaging, Inc., Thornwood, NY) equipped with the Zen pro 2.3 software was used to visualize the immunofluorescence staining for F4/80 and Ly-6C, and nuclear localization was provided by DAPI.
Article Title: Screening Linear and Circular RNA Transcripts from Stress Granules
Article Snippet: Cells were incubated with mouse anti-G3BP1 (Catalog No. 66486-1-Ig, Proteintech) primary antibody for 2 h at room temperature, followed by three times of washes with 1× PBS. .. Cells were then incubated with Alexa Fluor 555 goat anti-rabbit (Catalog No. 4413, Cell Signaling Technology, Beverly, MA) primary antibody for 2 h at room temperature, followed by three times of washes with 1× PBS. ..
Article Title: Screening Linear and Circular RNA Transcripts from Stress Granules.
Article Snippet: Cells were incubated with mouse anti-G3BP1 (Catalog No. 66486-1-Ig, Proteintech, Rosemont, IL) primary antibody for 2 h at room temperature, followed by three times washes with 1 × PBS. .. Cells were then incubated with Alexa Fluor 555 goat anti-rabbit (Catalog No. 4413, Cell Signaling Technology, Beverly, MA) primary antibody for 2 h at room temperature, followed by three times washes with 1 × PBS. ..
Sample Prep:Article Title: Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage
Article Snippet: .. Methodology Sample preparation Instrument type=Products&N=4294956287&Ntt=alexa+fluor+555+goat+anti-rat+&fromPage=plp&_requestid=60533 Alexa Fluor 555 goat anti-rabbit https://www.cellsignal.com/products/secondary-antibodies/anti-rabbit-igg-h-l-f-ab-2-fragmentalexa-fluor-555-conjugate/4413;jsessionid=gjul-ryLnG-3MkGVdqHFIzgK6_4dEEjEEqipJJSJ?site-searchtype=Products&N=4294956287&Ntt=alexa+fluor+555+goat+anti-rabbit+&fromPage=plp&_requestid=60616 rat anti-CD11b FITC-conjugated https://www.thermofisher.com/cn/zh/antibody/product/CD11b-Antibody-clone-M1-70Monoclonal/11-0112-82 rat anti-CD45 Percp Cy5.5-conjugated https://www.thermofisher.com/cn/zh/antibody/product/CD45-Antibody-clone-30-F11Monoclonal/45-0451-82 rat anti-CD16/32 APC-conjugated https://www.bdbiosciences.com/cn/applications/research/b-cell-research/surface-markers/ mouse/apc-rat-anti-mouse-cd16cd32-fc-iiiii-receptor-24g2/p/558636 rat anti-CD206 PE-conjugated https://www.thermofisher.com/cn/zh/antibody/product/CD206-MMR-Antibody-clone-MR6F3Monoclonal/12-2061-80 Rat IgG2b " Isotype control FITC-conjugated https://www.thermofisher.com/cn/zh/antibody/product/Rat-IgG2b-kappa-cloneeB149-10H5-Isotype-Control/11-4031-82 Rat IgG2a " Isotype control PerCP-Cy 5.5-conjugated https://www.thermofisher.com/cn/zh/antibody/product/Rat-IgG2a-kappaclone-eBR2a-Isotype-Control/45-4321-80 Rat IgG2b " Isotype control PE-conjugated https://www.thermofisher.com/cn/zh/antibody/product/Rat-IgG2b-kappa-cloneeB149-10H5-Isotype-Control/12-4031-82 Rat IgG2b " Isotype control APC- conjugated https://www.bdbiosciences.com/cn/reagents/research/antibodies-buffers/ immunology-reagents/anti-mouse-antibodies/cell-surface-antigens/apc-rat-igg2b-isotype-control-a95-1/p/553991 Specific pathogen-free, C57BL/6 male mice (6~8-week-old) were purchased from Shanghai Model Organisms Center. ..
Control:Article Title: Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage
Article Snippet: .. Methodology Sample preparation Instrument type=Products&N=4294956287&Ntt=alexa+fluor+555+goat+anti-rat+&fromPage=plp&_requestid=60533 Alexa Fluor 555 goat anti-rabbit https://www.cellsignal.com/products/secondary-antibodies/anti-rabbit-igg-h-l-f-ab-2-fragmentalexa-fluor-555-conjugate/4413;jsessionid=gjul-ryLnG-3MkGVdqHFIzgK6_4dEEjEEqipJJSJ?site-searchtype=Products&N=4294956287&Ntt=alexa+fluor+555+goat+anti-rabbit+&fromPage=plp&_requestid=60616 rat anti-CD11b FITC-conjugated https://www.thermofisher.com/cn/zh/antibody/product/CD11b-Antibody-clone-M1-70Monoclonal/11-0112-82 rat anti-CD45 Percp Cy5.5-conjugated https://www.thermofisher.com/cn/zh/antibody/product/CD45-Antibody-clone-30-F11Monoclonal/45-0451-82 rat anti-CD16/32 APC-conjugated https://www.bdbiosciences.com/cn/applications/research/b-cell-research/surface-markers/ mouse/apc-rat-anti-mouse-cd16cd32-fc-iiiii-receptor-24g2/p/558636 rat anti-CD206 PE-conjugated https://www.thermofisher.com/cn/zh/antibody/product/CD206-MMR-Antibody-clone-MR6F3Monoclonal/12-2061-80 Rat IgG2b " Isotype control FITC-conjugated https://www.thermofisher.com/cn/zh/antibody/product/Rat-IgG2b-kappa-cloneeB149-10H5-Isotype-Control/11-4031-82 Rat IgG2a " Isotype control PerCP-Cy 5.5-conjugated https://www.thermofisher.com/cn/zh/antibody/product/Rat-IgG2a-kappaclone-eBR2a-Isotype-Control/45-4321-80 Rat IgG2b " Isotype control PE-conjugated https://www.thermofisher.com/cn/zh/antibody/product/Rat-IgG2b-kappa-cloneeB149-10H5-Isotype-Control/12-4031-82 Rat IgG2b " Isotype control APC- conjugated https://www.bdbiosciences.com/cn/reagents/research/antibodies-buffers/ immunology-reagents/anti-mouse-antibodies/cell-surface-antigens/apc-rat-igg2b-isotype-control-a95-1/p/553991 Specific pathogen-free, C57BL/6 male mice (6~8-week-old) were purchased from Shanghai Model Organisms Center. ..
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